Cav1.3 Antibody

SKU: orb67391

Description

Mouse Anti-Rat Cav1.3 Monoclonal IgG2a Kappa

Research Area

Cancer Biology, Neuroscience, Pharmacology & Drug Discovery, Signal Transduction

Images & Validation

• Tested Applications: AM, ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), IHC-P (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-301 was sufficient for detection of Cav1.3 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG2a Kappa
• Clone No.: S48
• Immunogen: Fusion protein amino acids 859-875 of rat Cav1.3
• Target: Cav1.3
• Molecular Weight: 250kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Voltage-dependent L-type calcium channel subunit alpha-1D, Calcium channel, L type, alpha-1 polypeptide isoform 2, Voltage-gated calcium channel subunit alpha Cav1.3, CACNA1D, CACH3, CACN4, CACNL1A2, CCHL1A2, alpha-1 polypeptide, CAC1D_HUMAN, Cacna1d, Calcium channel, Calcium channel L type alpha 1 polypeptide isoform 2, Calcium channel neuroendocrine/brain type alpha 1 subunit, Calcium channel voltage dependent L type alpha 1D subunit, isoform 2, L type, Voltage dependent L type calcium channel subunit alpha 1D, Voltage gated calcium channel alpha 1 subunit, Voltage gated calcium channel alpha subunit Cav1.3

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav1.3 Monoclonal Antibody, Clone S48. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav1.3 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav1.3 Antibody (D) Composite.

Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody, Clone S48. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody, Clone S48. Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaV1.3 Calcium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Quick Database Links

Gene Symbol

Cav1.3

UniProt

P27732

NCBI Gene ID

29716

RefSeq (Protein):NP_058994.1