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Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:500-1:2000, IHC-P: 1:50-1:100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 75-190 of human Cathepsin G (NP_001902.1). |
| Molecular Weight | 29kDa |
| Purity | Affinity purification |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CG; CATG; Cathepsin G
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Immunohistochemistry analysis of paraffin-embedded human appendix using Cathepsin G Rabbit pAb (orb539066) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using Cathepsin G Rabbit pAb (orb539066) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human uterine cancer using Cathepsin G Rabbit pAb (orb539066) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using Cathepsin G Rabbit pAb (orb539066) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western blot analysis of various lysates using Cathepsin G Rabbit pAb (orb539066) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
VV Massoni, CMPC Silva, LDC Araujo, RB Lima Expression of NET markers in experimental apical periodontitis induced in mice Archives of Oral, (2025)
Cathepsin G Rabbit pAb (orb539066)
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