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Description
Research Area
Cancer Biology, Neuroscience, Signal Transduction
Images & Validation
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Item 1 of 1
| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100μl reaction mixture containing 25 mM MES, pH 5. 0, 0. 01 μg rhCathepsin B, 10 μM reaction substrate. The specific activity is >2800 pmol/min/μg. |
| Target | CTSB |
| Tag | His Tag |
| MW | 36.7 kDa (pro-form) and 29 kDa (mature-form) |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Arg 18 - Ile 339 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 50 mM Tris, 150 mM NaCl, pH 8.0 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CTSB, CPSB, APPS
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
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