Cathepsin B Recombinant Protein

SKU: orb1228094

Description

Cathepsin B Recombinant Protein

Research Area

Cancer Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: WB
• Application Notes:
  This recombinant protein can be used for WB. For research use only.

Key Properties

• Source: HEK293 cells
• Biological Activity: Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. Measured in 100μl reaction mixture containing 25 mM MES, pH 5. 0, 0. 01 μg rhCathepsin B, 10 μM reaction substrate. The specific activity is >2800 pmol/min/μg.
• Target: CTSB
• Tag: His Tag
• Molecular Weight: 36.7 kDa (pro-form) and 29 kDa (mature-form)
• Protein Sequence: Arg 18 - Ile 339
• Purity: >95% as determined by SDS-PAGE.

Storage & Handling

• Storage: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 50 mM Tris, 150 mM NaCl, pH 8.0
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CTSB, CPSB, APPS

Human Cathepsin B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Quick Database Links

Gene Symbol

CTSB

UniProt

P07858

RefSeq (Protein):NP_001899