Catalase Recombinant Mouse Monoclonal Antibody

SKU: orb1152050

Description

Catalase Recombinant Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG1, Kappa
• Clone No.: 4A8
• Immunogen: KLH conjugated synthetic peptide derived from human Catalase
• Target: CAT
• Molecular Weight: 60 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Cas1; CAT; Cs1; MGC138422; MGC138424; CATA_HUMAN.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:3000, Primary Ab incubation condition: room temperature 2h, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1:SH-SY5Y, 2: C2C12, 3: HepG2, 4: C6, Protein loading quantity: 20 µg, Exposure time: 60/10/60 s, Predicted MW: 60 kDa.

Paraformaldehyde-fixed, paraffin embedded (Human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (orb1152050) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (orb1152050) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (orb1152050) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human HepG2 cell lysates, Lane 3: Human 293T cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human Raji cell lysates, Primary: Anti-Catalase (orb1152050) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 60 kDa, Observed band size: 58 kDa.

Quick Database Links

Gene Symbol

CAT

UniProt

P04040