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Item 1 of 5
Catalase Recombinant Mouse Monoclonal Antibody
Catalog Number: orb1152050
| Catalog Number | orb1152050 |
|---|---|
| Category | Antibodies |
| Description | Catalase Recombinant Mouse Monoclonal Antibody |
| Target | CAT |
| Clonality | Recombinant |
| Species/Host | Mouse |
| Isotype | IgG1/Kappa |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide derived from human Catalase |
| UniProt ID | P04040 |
| MW | 60 kDa |
| Tested applications | IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:500-2000, IHC-P=1:20-100, IHC-F=1:20-100, IF=1:20-100 |
| Antibody Type | Recombinant Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Cas1; CAT; Cs1; MGC138422; MGC138424; CATA_HUMAN. |
| Note | For research use only |
Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:3000, Primary Ab incubation condition: room temperature 2h, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1:SH-SY5Y, 2: C2C12, 3: HepG2, 4: C6, Protein loading quantity: 20 µg, Exposure time: 60/10/60 s, Predicted MW: 60 kDa.
Paraformaldehyde-fixed, paraffin embedded (Human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (orb1152050) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (orb1152050) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Catalase) Monoclonal Antibody, Unconjugated (orb1152050) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human HepG2 cell lysates, Lane 3: Human 293T cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human Raji cell lysates, Primary: Anti-Catalase (orb1152050) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 60 kDa, Observed band size: 58 kDa.
