Cat IgG (H&L) Antibody Peroxidase Conjugated

SKU: orb346848

Description

Cat IgG (H&L) antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Reactivity: Feline
• Application Notes:
  This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Cat IgG whole molecule
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Cat IgG and Cat Serum.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Concentration: 2.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Goat anti-Cat IgG peroxidase Conjugated Antibody, Goat anti-Cat IgG HRP Conjugated Antibody

ELISA results using Goat Anti-Cat IgG HRP Evaluation of N(+RNA) and N(−RNA) proteins in the N-based ELISA using feline clinical serum samples. Seventeen seropositive (pos) and 14 seronegative (neg) cat serum samples were analyzed by N-based ELISA with either N(+RNA) and N(−RNA). The cutoff OD450 value is shown as a dashed line.

ELISA results using Goat Anti-Cat IgG HRP. ELISA reactivities against different antigens of pre–coronavirus disease (COVID-19) cat and dog serum samples and paired samples of FCoV type I infection, the Netherlands. A) Reactivities of pre–COVID-19 cat serum samples against SARS-CoV-2 S1, RBD, N, and FCoV type I S1. B) Reactivities of pre–COVID-19 dog serum samples against SARS-CoV-2 S1, RBD, N, BCoV S1, and FCoV type II S1. C) Reactivities of paired SPF cat serum samples (left panel) and FCoV type I–specific serum samples (right panel) to SARS-CoV-2 S1, subunit; RBD, N, and FCoV S1 protein levels were determined by ELISA. Dotted lines indicate positive cutoff levels. BCoV, bovine coronavirus; FCoV, feline coronavirus; N, nucleocapsid; OD, optical density; RBD, receptor-binding domain; S1, spike protein subunit 1; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; SPF, specific pathogen free.

ELISA results using Goat Anti-Cat IgG HRP. Serological tests of pet cat sera by ELISA. (a) Purified recombinant SARS-CoV-2 N and RBD proteins shown in SDS-PAGE gel after Coomassie blue staining. (b) A representative SARS-CoV-2 N IgG ELISA with pet cat sera. Normal cat serum purchased from a commercial source, the positive control (SARS-CoV N-specific mAb 1C7C7), and two seropositive samples (#29 and #11) are shown. (c) Pet cat sera tested by RBD IgG ELISA. The positive control (mAb 1C7C7), and seropositive samples are shown. (d) A batch of pet cat sera were tested with both N and RBD IgG ELISA. None of the RBD-positive sera are N-negative. The ID# of N seropositive samples are shown. (e) Evaluation of pet cat sera with IgG ELISA against feline infectious peritonitis virus (FIPV) antigens. Each serum was tested pairwise in uncoated and coated wells in technical duplicates. The adjusted OD450 value was calculated by subtracting OD450 value of uncoated well from that of the coated well. The cutoff OD450 value was calculated as described in Materials and Methods and shown as a red dash line.