CASPR Polyclonal Antibody

• Catalog Number: orb1421242
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CASPR.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Porcine, Rat
• Conjugation: Unconjugated
• UniProt ID: P78357
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Contactin-associated protein 1, Caspr, Caspr1, Neu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Tissue/cell: human glioma cells (U251 cells);4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CASPR Polyclonal Antibody, Unconjugated 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5ug/ml, blue) was used to stain the cell nuclei.

Blank control (Black line): HUVEC (Black).Primary Antibody (green line): Rabbit CASPR antibody.Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647. Dilution: 1 µg /test.Protocol. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20, 000 events was performed.