Caspase-9 (phospho Thr125) rabbit pAb

SKU: orb764344

Description

Rabbit polyclonal antibody to Caspase-9 (phospho-Thr125)

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 9 around the phosphorylation site of Thr125. AA range:91-140
• Molecular Weight: 42
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CASP9 antibody, anti-MCH6 antibody, anti-Caspase-9 antibody, anti-CASP-9 antibody, anti-Apoptotic protease Mch-6 antibody, anti-Apoptotic protease-activating factor 3 antibody, anti-APAF-3 antibody, anti-ICE-like apoptotic protease 6 antibody, anti-ICE-LAP6 antibody

Western Blot analysis of various cells using Phospho-Caspase-9 (T125) Polyclonal Antibody diluted at 1:500.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Caspase 9 (Phospho-Thr125) Antibody.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Caspase 9 (Phospho-Thr125) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with TNF 20ng/ml 5' + calyculinA 50ng/ml 5', using Caspase 9 (Phospho-Thr125) Antibody. The lane on the right is blocked with the phospho peptide.