Caspase-7 rabbit pAb

SKU: orb763897

Description

Rabbit polyclonal antibody to Caspase-7

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: WB 1:500-2000, IF 1:50-300, IHC 1:50-300
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthesized peptide derived from Caspase-7 . at AA range: 160-240
• Molecular Weight: 34
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CASP7 antibody, anti-MCH3 antibody, anti-Caspase-7 antibody, anti-CASP-7 antibody, anti-Apoptotic protease Mch-3 antibody, anti-CMH-1 antibody, anti-ICE-like apoptotic protease 3 antibody, anti-ICE-LAP3 antibody

Western Blot analysis of various cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800 (diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute? Plasma Membrane Protein Isolation and Cell Fractionation Kit (SM-005, Inventbiotech, MN, USA).

Immunohistochemical analysis of paraffin-embedded Human-breast tissue. 1, Caspase-7 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, Caspase-7 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, Caspase-7 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.