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Description
Research Area
Cell Biology
Images & Validation
−
| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:1000-5000, IHC-P=1:200-800, IHC-F=1:200-800, IF=1:200-800, Flow-Cyt=1μg/Test, ICC/IF=1:50-200 |
| Reactivity | Human |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone No. | B3D3 |
| Immunogen | Recombinant human Caspase-3 protein (29-277/277aa) |
| Target | CASP3 |
| Molecular Weight | 35(pro-Caspase3),15-17(Cleaved Caspase-3) kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CPP32; CPP32B; SCA-1; A830040C14Rik; AC-3; CASP-3; CC3; CPP-32; Caspase-3; Lice; Yama; mldy; CPP32-beta; CASP3_HUMAN; CASP3; Apopain; Cysteine protease CPP32 (CPP-32); Protein Yama; SREBP cleavage activity 1 (SCA-1); 3.4.22.56; CASP3_MOUSE; CASP3_PIG; CASP3_RABIT; Cysteine protease CPP32; CASP3_RAT; IRP; caspase 3; caspase 3, apoptosis-related cysteine protease; caspase 3, apoptosis-related cysteine peptidase
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Quick Database Links
Gene Symbol
CASP3
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry









