Caspase-12 Antibody (Large)

• Catalog Number: orb1239263
• Category: Antibodies
• Description: Caspase-12 Antibody (Large)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Caspase-12 antibody was raised against a synthetic peptide corresponding to 16 amino acids within the large cleavage product of murine caspase-12.The immunogen is located within amino acids 170 - 220 of Caspase-12.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 46 kDa Observed: 46 kDa
• Target: Casp12
• UniProt ID: O08736
• NCBI: CAA73532
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Large)
• Alternative names: Caspase-12 (Large) Antibody: Caspase-12, CASP-12
• Note: For research use only
• Application notes: Caspase-12 (large) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 0.25 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human, mouse, and rat samples and Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence Validation of Caspase-12 in Mouse Heart. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart labeling caspase-12 with orb1239263 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).

Immunohistochemistry Validation of Caspase-12 in Human Colon. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Caspase-12 antibody (orb1239263) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of Caspase-12 in Mouse Brain. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Caspase-12 antibody (orb1239263) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of Caspase-12 in Rat Brain. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Caspase-12 antibody (orb1239263) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

WB Validation in Human and Mouse Heart. Loading: 15 µg of lysate Antibodies: Caspase-12, orb1239263, 0.5 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Lane A: Human heart Lane B: Mouse heart.

WB Validation in Human Tissues. Loading: 15 µg of lysate Antibodies: Caspase-12, orb1239263, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Rat Tissues. Loading: 15 µg of lysate Antibodies: Caspase-12, orb1239263, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.