CASP1 Antibody

SKU: orb1261749

Description

CASP1 Antibody

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-311 of human Caspase 1 (NP_150635.1).
• Target: CASP1
• Molecular Weight: Observed: 30kDa/48kDa
• Purification: Affinity purification

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Caspase-1 Antibody: ICE, P45, IL1BC, IL1BCE, Caspase-1, Interleukin-1 beta convertase, CASP-1

Western blot analysis of extracts of various cell lines, using Caspase 1 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human liver cancer using Caspase 1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using Caspase 1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using Caspase 1 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of Raw264. 7 cells using Caspase 1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of THP-1 cells, using 3 ug Caspase 1 antibody. Western blot was performed from the immunoprecipitate using Caspase 1 antibody at a dilution of 1:1000.

Quick Database Links

Gene Symbol

CASP1

UniProt

P29466