CASP1 Antibody

SKU: orb1240049

Description

CASP1 Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Predicted Reactivity: Equine, Porcine
• Application Notes:
  Casp-1 antibody can be used for the detection of Caspase-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Caspase-1 antibody was raised against a 15 amino acid synthetic peptide from near the middle of human Caspase-1.The immunogen is located within amino acids 200 - 250 of Caspase-1.
• Target: CASP1
• Purification: Caspase-1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Caspase-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

Caspase-1 Antibody: ICE, P45, IL1BC, IL1BCE, Caspase-1, Interleukin-1 beta convertase, CASP-1

Western blot analysis of Caspase-1 in HeLa cell lysate with Caspase-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 µg/mL.

Immunohistochemistry of caspase-1 in human spleen tissue with caspase-1 antibody at 10 µg/mL.

Immunofluorescence of Caspase-1 in Hela cells with Caspase-1 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

CASP1

UniProt

P29466

RefSeq (Protein):NP_150634