CASP8 Antibody

SKU: orb625440

Description

Mouse monoclonal antibody to Caspase 8

Research Area

Apoptotic, Cancer, Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IF, IHC, IP, WB
• Dilution range: WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:100-1:400; IF: 1:50-1:500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Immunogen: caspase 8, apoptosis-related cysteine peptidase
• Target: CASP8
• Molecular Weight: 50-55 kDa, 25-32 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Protein A+G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Disclaimer: For research use only

Alternative Names

Apoptotic cysteine protease, Apoptotic protease Mch 5, Casp 8, CASP8, Caspase 8, Caspase 8/p18, Caspase8, FADD like ICE, FLICE, ICE like apoptotic protease 5, MACH, MCH5

Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using orb625440 (Caspase 8 Antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

IP Result of anti-Caspase 8 (IP: orb625440, 5 ug; Detection: orb625440 1:500) with HepG2 cells lysate 2400 ug.

HepG2 cells were subjected to SDS PAGE followed by western blot with orb625440 (CASP8 antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using orb625440 (Caspase 8 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9).

Quick Database Links

Gene Symbol

CASP8

UniProt

Q14790