CASKIN2 Antibody

• Catalog Number: orb1539398
• Category: Antibodies
• Description: CASKIN2 Antibody
• Target: CASKIN2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Purification: Peptide Column Affinity Chromatography
• Immunogen: A 19 AA synthetic peptide near the N-Terminus of human CASKIN2. The immunogen is located within aa 380-430 of CASKIN2.
• Tested applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Dilution range: ELISA, ICC (2.5 µg/ml), IF (5 µg/ml), IHC, IHC-P (5 µg/ml), WB (1 µg/ml)
• Application notes: Further information: CASKIN2 antibody can be used for detection of EPAC1 by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 123, 132 kDa Observed: 120 kDa
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CASKIN2, ANKS5B, CASK interacting protein 2, Caski
• Note: For research use only

Anti-CASKIN2 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunocytochemistry of CASKIN2 in HeLa cells with CASKIN2 antibody at 2.5 µg/mL.

Immunofluorescence of CASKIN2 in HeLa cells with CASKIN2 antibody at 20 µg/mL.

Western blot analysis of CASKIN2 in HeLa cell lysate with CASKIN2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.