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Cas 9 Antibody Peroxidase Conjugated
Catalog Number: orb420462
| Catalog Number | orb420462 |
|---|---|
| Category | Antibodies |
| Description | Cas 9 antibody (Peroxidase) |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | HRP |
| Reactivity | Bacteria |
| Form/Appearance | Lyophilized |
| Concentration | 1.0mg/mL |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Purity | Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. |
| Immunogen | Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein. |
| UniProt ID | Q99ZW2 |
| Tested applications | ELISA, IF, WB |
| Dilution range | ELISA: 1:25,000-1:30,000, IF: 1µg/mL, WB: 1µg/mL |
| Application notes | Cas9 Peroxidase Conjugated Antibody has been tested for use in ELISA and suitable in Immunofluorescence and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract. |
| Antibody Type | Primary Antibody |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Alternative names | rabbit anti-Cas9 antibody peroxidase conjugation, Read more... |
| Note | For research use only |
| NCBI | NP_269215.1 |
Immunofluorescence of Rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1 µg/ml. Secondary: Donkey anti-Rabbit at 1 µg/ml, staining seen in red. Bottom images show GFP stain overlay.
Western Blot of Rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15 µg per lane. Primary Antibody cas9 used at 1 µg/ml using orb348637 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40000 for 30 min at room temp. Expect: 158kDa.
