CTF1 Antibody

SKU: orb1272581

Description

CTF1 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Human
• Application Notes:
  Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hCardiotrophin 1 (15 ng/mL), a concentration of 0.25 - 0.40 μg/mL of this antibody is required. ELISA:To detect hCardiotrophin 1 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hCardiotrophin 1. Western Blot:To detect hCardiotrophin 1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCardiotrophin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCardiotrophin 1 (human Cardiotrophin-1).
• Target: CTF1
• Purification: Anti-hCardiotrophin 1 specific antibody was purified by affinity chromatography employing immobilized hCardiotrophin 1 matrix.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CT1, CT-1Cardiotrophin-1CT-1

To detect hCT-1 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human CT-1 (orb1272580) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCT-1.

This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

CTF1

UniProt

Q16619

RefSeq:Q16619