TBC1D10C Antibody

SKU: orb1246743

Description

TBC1D10C Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 48-50kDa band observed in lysates of cell line Jurkat and in Mouse Thymus lysates and approx 50kDa in Peripheral Blood Mononuclear (PBM) and in Human Spleen lysates (calculated MW of 49.7Da according to Human NP_940919.1 and 49.9kDa according to Mouse NP_848765.2). Recommended concentration:0.3-2µg/mL. Primary incubation 1 hour at room temperature. Negative Control: A431 cell lysate.Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-PRRKPQTRGKTFH
• Target: TBC1D10C
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TBC1D10C, carabin, TBC1 domain family, member 10C, FLJ00332, MGC46488

orb1246743 (0.5 ug/ml) staining of Jurkat (A), (0.3 ug/ml) PBM (B) and negative control A431 (C) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246743 (2 ug/ml) staining of Human Spleen (A) and Mouse Thymus (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246743 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).

Quick Database Links

Gene Symbol

TBC1D10C

UniProt

Q8IV04

RefSeq (Protein):NP_940919.1