You have no items in your shopping cart.
Cart summary
Item 1 of 4
Item 1 of 4
CAPZA1 Antibody (N-term)
Catalog Number: orb1929214
| Catalog Number | orb1929214 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB17974 |
| Tested applications | FC, IF, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:4000, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 32923 Da |
| Target | This CAPZA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CAPZA1. |
| UniProt ID | P52907 |
| NCBI | NP_006126.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | F-actin-capping protein subunit alpha-1, CapZ alph Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of CAPZA1 antibody (N-term) in HL60 cell line lysates (35 ug/lane). CAPZA1 (arrow) was detected using the purified Pab.
CAPZA1 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescence analysis of CAPZA1 Antibody (N-term) with paraffin-embedded human prostate carcinoma tissue.0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
All lanes: Anti-CAPZA1 Antibody (N-term) at 1:4000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 33 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
