CAPRIN1 Antibody

SKU: orb1248095

Description

CAPRIN1 Antibody

Research Area

Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 80+140kDa bands observed in lysates of cell line Caco-2, HeLa, Jurkat and K562 (calculated MW of 78.4kDa according to NP_005889.3). The ~140kDa band corresponds to the reported p137 (Ellis and Luzio, J Biol Chem. 1995 Sep 1;270(35):20717-23.PMID: 7657653). Recommended concentration: 0.3-1µg/mL.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-EQRPQKEPIDQ
• Target: CAPRIN1
• Molecular Weight: Approx 80 + 140kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CAPRIN1, cell cycle associated protein 1, GPIAP1, GPIP137, M11S1, RNG105, p137GPI, GPI-anchored membrane protein 1, GPI-anchored protein p137, GPI-p137, RNA granule protein 105, activation/proliferation-associated protein 1, caprin 1, caprin-1, cell cycle

orb1248095 (0.3 ug/ml) staining of Jurkat (A) and HeLa (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248095 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

CAPRIN1

UniProt

Q14444

RefSeq (Protein):NP_976240.1, NP_005889.3