CAMK2 beta Antibody (C-term)

SKU: orb1925571

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Immunology & Inflammation, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:25
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: B3272EV36X3X5
• Immunogen: This CAMK2 beta antibody is generated from a mouse immunized with a recombinant protein between 1-503 amino acids from human CAMK2 beta.
• Target: CAMK2B
• Molecular Weight: 72678 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Calcium/calmodulin-dependent protein kinase type II subunit beta, CaM kinase II subunit beta, CaMK-II subunit beta, 2.7.11.17, CAMK2B, CAM2, CAMK2, CAMKB

All lanes: Anti-CAMK2 beta Antibody (C-term) at 1:2000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: PC-12 whole cell lysate. Lane 3: human brain lysate. Lane 4: mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 60 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining CAMK2 in human cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Quick Database Links

Gene Symbol

CAMK2B

UniProt

Q13554