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CALU Antibody

SKU: orb1256948
FeaturedFeatured Product

Description

CALU Antibody

Research Area

Neuroscience, Signal Transduction

Images & Validation

Tested ApplicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
Application Notes
WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-315 of human CALU (NP_001210.1).
TargetCALU
Molecular WeightObserved: 50kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentrationbatch dependent
DisclaimerFor research use only

Alternative Names

Calumenin, Crocalbin, IEF SSP 9302, CALU

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CALU Antibody

Western blot analysis of extracts of various cell lines, using CALU antibody (orb1256948) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

CALU Antibody

Immunohistochemistry of paraffin-embedded mouse skin using CALU antibody (orb1256948) at dilution of 1:100 (40x lens).

CALU Antibody

Immunohistochemistry of paraffin-embedded rat lung using CALU antibody (orb1256948) at dilution of 1:100 (40x lens).

CALU Antibody

Immunohistochemistry of paraffin-embedded mouse lung using CALU antibody (orb1256948) at dilution of 1:100 (40x lens).

CALU Antibody

Immunofluorescence analysis of NIH-3T3 cells using CALU antibody (orb1256948) at dilution of 1:100. Blue: DAPI for nuclear staining.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol

CALU Antibody (orb1256948)

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Available Sizes

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100 μl
$ 830.00
DispatchUsually dispatched within 5-10 working days
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