CALR Antibody

• Catalog Number: orb1261367
• Category: Antibodies
• Description: CALR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 18-417 of human CALR (NP_004334.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:100IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 55kDa
• Target: CALR
• UniProt ID: P27797
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: CALR, RO, CRT, SSA, cC1qR, HEL-S-99n
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CALR antibody at 1:1000 dilution.

Immunofluorescence analysis of HeLa cells using CALR antibody. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using CALR antibody at dilution of 1:400. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U-2OS cells using CALR antibody at dilution of 1:400. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug CALR antibody. Western blot was performed from the immunoprecipitate using CALR antibody at a dilution of 1:1000.