Calprotectin Antibody

SKU: orb1539483

Description

Calprotectin Antibody

Images & Validation

• Tested Applications: IHC, IHC-P
• Dilution Range: IHC, IHC-P (1:50)
• Reactivity: Canine, Feline, Guinea pig, Human, Monkey, Mouse, Porcine, Primate, Rabbit, Rat
• Application Notes:
  Further information: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. This antibody may be used at a dilution 1:50 in IHC. Predigestion with trypsin @ 1 mg/ml PBS, 10 mins. at 37 C. or a high temperature antigenic unmasking technique (boiling tissue sections in 10 mM citrate, pH 6.0 for 10-20 mins., folowed by cooling to RT for 10-20 mins.)) is required.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: MAC387
• Immunogen: Affinity purified monocyte membrane preparation
• Target: Calprotectin
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 20 mM Tris-borate, 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM, Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, 0.05% sodium azide, pH 7.5
• Concentration: 0.4 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-Calprotectin antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

Quick Database Links

Gene Symbol

Calprotectin