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Item 1 of 4
Item 1 of 4
Calponin-1 Antibody (N-term)
Catalog Number: orb1929801
| Catalog Number | orb1929801 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB19472 |
| Tested applications | FC, IHC-P, WB |
| Predicted Reactivity | Mouse, Rat, Sheep |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 33170 Da |
| Target | This Calponin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-39 amino acids from the N-terminal region of human Calponin-1. |
| UniProt ID | P51911 |
| NCBI | NP_001290.2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Calponin-1, Basic calponin, Calponin H1, smooth mu Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of NCI-H292 cells using Calponin-1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human lung carcinoma with Calponin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of Calponin-1 Antibody (N-term) in NCI-H460 cell line lysates (35 ug/lane). CNN1 (arrow) was detected using the purified Pab.
Western blot analysis of lysates from Hela, MDA-MB-453 cell line (from left to right), using Calponin-1 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20 ug per lane.
