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Catalog Number | orb151327 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody against Calnexin conjugated to FITC |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC |
Reactivity | Aves, Bovine, Canine, Drosophila, Frog, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep |
Immunogen | Dog calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues. |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000), IHC (1:100), ICC/IF (1:100) |
Conjugation | FITC |
MW | 90kDa |
Target | Calnexin |
Entrez | 403908 |
UniProt ID | P24643 |
NCBI | NP_001003232.1 |
Storage | Conjugated antibodies should be stored according to the product label |
Buffer/Preservatives | 640.91mM DMSO, 136.36mM Ethanolamine, and 9.09mM Sodium Bicarbonate in 90.9% PBS |
Alternative names | Calnexin antibody, CALX_HUMAN antibody, CANX antib Read more... |
Note | For research use only |
Application notes | A 1:1000 dilution of SPC-108 was sufficient for detection of Calnexin in 10 µg of HeLa cell lysate by ECL immunoblot analysis. |
Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody at 1:1000.
Immunohistochemistry analysis using Rabbit Anti-Calnexin Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT.
Western blot analysis of Rat Tissue lysates showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody. Load: 15 μg protein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
ICC, IF, IHC | |
Aves, Bovine, Canine, Drosophila, Frog, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep | |
Rabbit | |
Polyclonal | |
FITC |