Calnexin-CT Antibody: Biotin

• Catalog Number: orb151326
• Category: Antibodies
• Description: Rabbit polyclonal antibody against Calnexin conjugated to Biotin
• Target: Calnexin
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Biotin
• Reactivity: Aves, Bovine, Canine, Drosophila, Frog, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
• Concentration: 1 mg/ml
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Purification: Protein A purified
• Immunogen: Dog calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
• UniProt ID: P24643
• MW: 90kDa
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000), IHC (1:100), ICC/IF (1:100)
• Application notes: A 1:1000 dilution of SPC-108 was sufficient for detection of Calnexin in 10 µg of HeLa cell lysate by ECL immunoblot analysis.
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: Calnexin antibody, CALX_HUMAN antibody, CANX antib
• Note: For research use only
• Entrez: 403908
• NCBI: NP_001003232.1

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody at 1:1000.

Immunohistochemistry analysis using Rabbit Anti-Calnexin Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT.

Western blot analysis of Rat Tissue lysates showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody. Load: 15 μg protein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.