Calnexin-CT Antibody (APC)

SKU: orb151325

Description

Rabbit polyclonal antibody against Calnexin conjugated to APC

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution range: WB (1:1000), IHC (1:100), ICC/IF (1:100)
• Reactivity: Aves, Bovine, Canine, Drosophila, Frog, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
• Application Notes:
  A 1:1000 dilution of SPC-108 was sufficient for detection of Calnexin in 10 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Dog calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
• Target: Calnexin-CT
• Molecular Weight: 90kDa
• Purification: Protein A purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Calnexin, CALX_HUMAN, CANX, CNX, FLJ26570, IP90, p88, p90, Histocompatibility complex class I antigen binding protein p88, Major histocompatibility complex class I antigen-binding protein p88

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody at 1:1000.

Immunohistochemistry analysis using Rabbit Anti-Calnexin Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT.

Western blot analysis of Rat Tissue lysates showing detection of Calnexin protein using Rabbit Anti-Calnexin Polyclonal Antibody. Load: 15 μg protein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol

Calnexin-CT

UniProt

P24643

NCBI Gene ID

403908

RefSeq (Protein):NP_001003232.1