Calmodulin rabbit pAb Antibody

SKU: orb764704

Description

Rabbit polyclonal antibody to Calmodulin

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Calmodulin. AA range:46-95
• Molecular Weight: 17kD
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CALM1 antibody, anti-CALM antibody, anti-CAM antibody, anti-CAM1 antibody, anti-CALM2 antibody, anti-CAM2 antibody, anti-CAMB antibody, anti-CALM3 antibody, anti-CALML2 antibody, anti-CAM3 antibody, anti-CAMC antibody, anti-CAMIII antibody, anti-Calmodulin antibody, anti-CaM antibody

Immunofluorescence analysis of Hela cell. 1, Calmodulin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min).

Western blot analysis of lysates from 1) HuvEc, 2) HepG2, 3) 293 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) Actin β Monoclonal Antibody (5B7) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour.

Western Blot analysis of various cells using Calmodulin Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of HuvEc cells using Calmodulin Polyclonal Antibody diluted at 1:2000.