Calmodulin Antibody (C-term)

SKU: orb1939613

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:250
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Other, Rabbit, Sheep, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This Calmodulin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-149 amino acids from the C-terminal region of human Calmodulin. Antigen Region: 117-149 aa.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Calmodulin, CaM, CALM1, CALM, CAM, CAM1

All lanes: Anti-Calmodulin Antibody at 1:2000 dilution. Lane 1: SH-SY5Y whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: Mouse brain lysate. Lane 4: Rat brain lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-hCalmodulin-D132 at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: SH-SY5Y whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: Rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining hCalmodulin-D132 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.

Quick Database Links

UniProt

P62158

RefSeq (Protein):NP_008819.1, NP_005175.2, NP_001734.1