Caldesmon / CAD Antibody HMW

• Catalog Number: orb386230
• Category: Antibodies
• Description: Caldesmon (CAD, CALD, CALD1) is an actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). [UniProt]
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CALD1/1424R
• Tested applications: IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG, kappa
• Immunogen: A full length human protein was used as the immunogen for the recombinant Caldesmon antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Caldesmon antibody is available for research use only.
• UniProt ID: Q05682
• Storage: Store the recombinant Caldesmon antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the recombinant Caldesmon antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human ovary tissue lysate using recombinant Caldesmon antibody (clone CALD1/1424R). Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).

IHC testing of FFPE human uterus with recombinant Caldesmon antibody (clone CALD1/1424R). Required HIER: boil tissue sections in 1 mM EDTA, pH9, for 10-20 min.

IHC testing of FFPE rat uterus with recombinant Caldesmon antibody (clone CALD1/1424R). Required HIER: boil tissue sections in 1 mM EDTA, pH9, for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Caldesmon antibody (clone CALD1/1424R) as confirmation of integrity and purity.