Recombinant Caldesmon Antibody / CAD / CALD1 (HMW)

SKU: orb2637256

Description

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant Caldesmon antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rCALD1/820
• Immunogen: Recombinant full-length human CALD1 protein was used as the immunogen for the recombinant Caldesmon antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of human ovary tissue lysate using recombinant Caldesmon antibody (clone rCALD1/820). Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).

IHC staining of FFPE human leiomyosarcoma tissue with recombinant Caldesmon antibody (clone rCALD1/820). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Caldesmon antibody (clone rCALD1/820) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q05682