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Catalog Number | orb1930403 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB22504 |
Tested applications | FC, IF, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Dilution range | IF: 1:10~50, WB: 1:1000, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 55345 Da |
Target | This CALCR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-460 amino acids of human CALCR. |
UniProt ID | P30988 |
NCBI | NP_001158210.1, NP_001733.1, NP_001158209.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Calcitonin receptor, CT-R, CALCR Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
CALCR Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of CALCR Antibody (C-term) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of lysate from MCF-7, A431 cell line, using CALCR Antibody (C-term) with (+) or without (-) peptides. Diluted at 1:1000 at each lane. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug per lane.