Calcium Sensing Receptor Antibody (Biotin)

SKU: orb384333

Description

Rabbit polyclonal antibody against CASR conjugated to Biotin.

Research Area

Cancer Biology, Cell Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human
• Application Notes:
  A 1:1000 dilution was sufficient for detection of calcium-sensing receptor (CASR) in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the N-terminal (1-100 aa) of Human calcium-sensing receptor (CASR)
• Target: Calcium Sensing Receptor
• Molecular Weight: 140 kDa
• Purification: Peptide Affinity Purified
• Conjugation: Biotin

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Calcium-Sensing Receptor, CASR, CASR_HUMAN, CaSR, Ca2+ sensing receptor, Ca2+ sensing receptor 1, Ca sensing receptor, Extracellular calcium-sensing receptor, Extracellular calcium sensing receptor, Extracellular calcium sensing receptor [Precursor], Parathyroid cell calcium-sensing receptor, Parathyroid Cell calcium sensing receptor, Parathyroid Cell calcium-sensing receptor 1, Parathyroid Ca(2+) sensing receptor 1, PCAR1, PCaR1, PCAR 1, GPRC2A, CAR, HHC, HHC1, FHH, FIH, NSHPT, EIG8, MGC138441, Hypocalciuric hypercalcemia 1, Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Calcium Sensing Receptor Antibody (D) Composite.

Western blot analysis of Human HeLa cell lysates showing detection of 130kDa Calcium Sensing Receptor protein using Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 130kDa.

Quick Database Links

Gene Symbol

Calcium Sensing Receptor

UniProt

P41180

NCBI Gene ID

846

RefSeq (Protein):NP_000379.2