Calbindin 1 antibody

SKU: orb2276326

Description

Calbindin 1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:50-1:100
• Reactivity: Human
• Application Notes:
  Positive Control: Kidney: A subset of the cells of distal tubuli should show an intense cytoplasmic calbindin 1 immunostaining. Negative Control: Colon: Calbindin 1 immunostaining should be completely absent in all cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

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Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2c, kappa
• Clone No.: MSVA-471M
• Immunogen: Recombinant fragment (around aa 7-96) of human CALB1 protein (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

avian-type; CAB27; CALB 1; CALB; CALB1; CALB1_HUMAN; Calbindin 1 28kDa; Calbindin; Calbindin D28; D 28K; D-28K; D28K; OTTHUMP00000166027; OTTHUMP00000225441; RTVL H protein; Vitamin D dependent calcium binding protein; Vitamin D dependent calcium binding protein avian type; Vitamin D-dependent calcium-binding protein

Quick Database Links

UniProt

P05937