Calbindin 1 antibody (MSVA-471M)

• Catalog Number: orb2276326
• Category: Antibodies
• Description: Calbindin 1 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-471M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG2c, kappa
• Immunogen: Recombinant fragment (around aa 7-96) of human CALB1 protein (exact sequence is proprietary)
• Dilution range: 1:50-1:100
• Conjugation: Unconjugated
• UniProt ID: P05937
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: avian-type; CAB27; CALB 1; CALB; CALB1; CALB1_HUMA
• Note: For research use only
• Application notes: Positive Control: Kidney: A subset of the cells of distal tubuli should show an intense cytoplasmic calbindin 1 immunostaining. Negative Control: Colon: Calbindin 1 immunostaining should be completely absent in all cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Cerebellum molecular layer Purkinje cell layer granule cell layer white matter A strong Calbindin 1 staining of Purkinje cells and of associated axonal fibres is seen in the cerebrum.

Colon descendes mucosa Calbindin 1 immunostaining is absent in all cells of the colon mucosa.

Kidney cortex A strong cytoplasmic Calbindin 1 immunostaining occurs in a fraction of distal tubuli of the kidney.