CAF1p55/VPRBP Polyclonal Antibody

SKU: orb1416395

Description

Rabbit polyclonal antibody to CAF1p55/VPRBP.

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Reactivity: Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Disclaimer: For research use only

Alternative Names

DDB1- and CUL4-associated factor 1 {ECO:0000312|HGNC:HGNC:30911}, HIV-1 Vpr-binding protein, VprBP, Serine/threonine-protein kinase VPRBP, 2.7.11.1, Vpr-interacting protein, DCAF1 (HGNC:30911), KIAA0800, RIP, VPRBP

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CAF1p55/VPRBP) Polyclonal Antibody, Unconjugated at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Blank control (Black line): Molt4 (Black).Primary Antibody (green line): Rabbit Anti-CAF1p55_VPRBP antibody.Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647. Dilution: 1 µg /test.Protocol. The cells were fixed with 4% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20, 000 events was performed.

Quick Database Links

UniProt

Q9Y4B6