CADM4 Antibody

SKU: orb1247711

Description

CADM4 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 70kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 42.8kDa according to NP_660339.1). The observed molecular weight corresponds to findings with EB08040, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.03-0.1µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen and Liver. Recommended concentration: 2.5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-REAFLNGSDGHKRKE
• Target: CADM4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CADM4, cell adhesion molecule 4 , IGSF4C, Necl-4, TSLL2, synCAM4, TSLC1-like 2, immunoglobulin superfamily, member 4C, nectin-like 4

Staining of Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer). A) EB08040 (0.1 ug/ml) and B) orb1247711 (0.05 ug/ml). Detected by chemiluminescence.

orb1247711 (2.5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247711 (2.5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

CADM4

UniProt

Q8NFZ8

RefSeq (Protein):NP_660339.1