CADM4 Antibody

• Catalog Number: orb1246744
• Category: Antibodies
• Description: CADM4 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-KDERFQLEEFS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 70kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 42.8kDa according to NP_660339.1). The observed molecular weight corresponds to findings with EB08194, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thymus and Skeletal Muscle. Recommended concentration: 2.5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CADM4
• UniProt ID: Q8NFZ8
• NCBI: NP_660339.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CADM4, cell adhesion molecule 4, IGSF4C, Necl-4, T
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 70kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 42.8kDa according to NP_660339.1). The observed molecular weight corresponds to findings with EB08194, a product of different design reacting to a different part of the same protein. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thymus and Skeletal Muscle. Recommended concentration: 2.5ug/ml.
• Expiration Date: 12 months from date of receipt.

Staining of Human Brain (Cerebellum) lysate (35 ug protein in RIPA buffer). A) orb1246744 (0.1 ug/ml) and B) orb1247711 (0.05 ug/ml). Detected by chemiluminescence.

orb1246744 (2.5 ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246744 (2.5 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.