CACNA2D2 Antibody (Center)

SKU: orb1936716

Description

CACNA2D2 Antibody (Center)

Research Area

Cell Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution range: WB - 1:2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 129817 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

KIAA0558

Anti-CACNA2D2 Antibody (Center) at 1:2000 dilution + human testis lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 130 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-CACNA2D2 Antibody (Center) at 1:2000 dilution + mouse kidney lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 130 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

CACNA2D2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CACNA2D2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Fluorescent image of U251 cell stained with CACNA2D2 Antibody (Center).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CACNA2D2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).CACNA2D2 immunoreactivity is localized to Cytoplasm significantly.

Quick Database Links

UniProt

Q9NY47