CACNA2D2 Antibody (Center)

• Catalog Number: orb1936716
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB32703
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, WB: 1:2000, IHC-P: 1:10~50, IF: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 129817 Da
• Target: This CACNA2D2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 643-671 amino acids from the Central region of human CACNA2D2.
• UniProt ID: Q9NY47
• NCBI: NP_001005505.1, NP_006021.2, NP_001167522.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Voltage-dependent calcium channel subunit alpha-2/
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-CACNA2D2 Antibody (Center) at 1:2000 dilution + human testis lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 130 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-CACNA2D2 Antibody (Center) at 1:2000 dilution + mouse kidney lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 130 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

CACNA2D2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of CACNA2D2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Fluorescent image of U251 cell stained with CACNA2D2 Antibody (Center).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CACNA2D2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C).CACNA2D2 immunoreactivity is localized to Cytoplasm significantly.