CA9 Antibody / Carbonic Anhydrase IX

• Catalog Number: orb2635849
• Category: Antibodies
• Description: CAIX is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, CAIX is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CA9/9621R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: The extracellular N-terminal proteoglycan-like region was used as the immunogen for the recombinant Carbonic Anhydrase IX antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This recombinant Carbonic Anhydrase IX antibody is available for research use only.
• UniProt ID: Q16790
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Carbonic Anhydrase IX antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human HeLa cell lysate with recombinant Carbonic Anhydrase IX antibody. Predicted molecular weight: 50-55 kDa but may be observed at higher molecular weights due to glycosylation.

Western blot testing of human stomach tissue lysate with recombinant Carbonic Anhydrase IX antibody. Predicted molecular weight: 50-55 kDa but may be observed at higher molecular weights due to glycosylation.

IHC staining of FFPE human renal cell carcinoma tissue with Carbonic Anhydrase IX antibody (clone CA9/9621R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Carbonic Anhydrase IX antibody (clone CA9/9621R) as confirmation of integrity and purity.