CA5B Antibody (C-term)

SKU: orb1929376

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB18377
• Immunogen: This CA5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-283 amino acids from the C-terminal region of human CA5B. Antigen Region: 253-283 aa.
• Target: CA5B
• Molecular Weight: 36434 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Carbonic anhydrase 5B, mitochondrial, Carbonate dehydratase VB, Carbonic anhydrase VB, CA-VB, CA5B

CA5B Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

CA5B Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CA5B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of CA5B Antibody (C-term) in mouse liver tissue lysates (35 ug/lane). CA5B (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

CA5B

UniProt

Q9Y2D0

RefSeq (Protein):NP_009151.1