CA3 Antibody / Carbonic Anhydrase III

• Catalog Number: orb1822869
• Category: Antibodies
• Description: Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA III (carbonic anhydrase III), also known as Car3 or CA3, is a 260 amino acid cytoplasmic protein that is specifically expressed in muscle. Belonging to the a-carbonic anhydrase family, CA III is activated by proton donors such as imidazole and dipeptide histidylhistidine, and is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant fragment of human protein was used as the immunogen for the Carbonic Anhydrase 3 antibody.
• UniProt ID: P07451
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Antibody Type: Primary Antibody
• Clone Number: CA3/7883
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Carbonic Anhydrase 3 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human skeletal muscle tissue with Carbonic Anhydrase 3 antibody (clone CA3/7883). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human RH-30 cell lysate with Carbonic Anhydrase 3 antibody (clone CA3/7883). Predicted molecular weight ~29 kDa.

SDS-PAGE analysis of purified, BSA-free Carbonic Anhydrase 3 antibody (clone CA3/7883) as confirmation of integrity and purity.