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CA3 Antibody / Carbonic Anhydrase III

Catalog Number: orb1822862

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb1822862
CategoryAntibodies
DescriptionCarbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA III (carbonic anhydrase III), also known as Car3 or CA3, is a 260 amino acid cytoplasmic protein that is specifically expressed in muscle. Belonging to the a-carbonic anhydrase family, CA III is activated by proton donors such as imidazole and dipeptide histidylhistidine, and is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman
ImmunogenA recombinant fragment of human CA3 protein was used as the immunogen for the CA3 antibody.
UniProt IDP07451
Tested applicationsIHC-P, WB
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Antibody TypePrimary Antibody
Clone NumberCA3/7885
Formula1 mg/ml in 1X PBS; BSA free, sodium azide free
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis CA3 antibody is available for research use only.
NoteFor research use only
CA3 Antibody / Carbonic Anhydrase III

IHC staining of FFPE human skeletal muscle tissue with CA3 antibody (clone CA3/7885). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

CA3 Antibody / Carbonic Anhydrase III

IHC staining of FFPE human skeletal muscle tissue with CA3 antibody (clone CA3/7885). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

CA3 Antibody / Carbonic Anhydrase III

Western blot testing of human RH30 cell lysate with CA3 antibody (clone CA3/7885). Predicted molecular weight ~29 kDa.

CA3 Antibody / Carbonic Anhydrase III

SDS-PAGE analysis of purified, BSA-free CA3 antibody (clone CA3/7885) as confirmation of integrity and purity.

  • CA3 Antibody / Carbonic Anhydrase III [orb1822863]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg, 20 μg
  • CA3 Antibody / Carbonic Anhydrase III [orb1822868]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • CA3 Antibody / Carbonic Anhydrase III [orb1822869]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg
  • CA3 Antibody / Carbonic Anhydrase III [orb1822865]

    IHC-P

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • CA3 Antibody / Carbonic Anhydrase III [orb1822866]

    IHC-P

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg, 20 μg