CA2 Antibody (N-term)

SKU: orb1929378

Description

CA2 Antibody (N-term)

Research Area

Cardiovascular Research, Cell Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB - 1:1000, IF - 1:10-50
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 29246 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Fluorescent confocal image of Hela cell stained with CA2 Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CA2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). CA2 immunoreactivity is localized to Cytoplasm significantly.

CA2 Antibody (N-term) western blot analysis in MCF-7 cell line and mouse kidney tissue lysates (35 ug/lane). This demonstrates the CA2 antibody detected the CA2 protein (arrow).

Western blot analysis of lysates from MCF-7 cell line and mouse kidney tissue lysate (from left to right), using CA2 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Quick Database Links

UniProt

P00918