CA-5f

SKU: orb1302020

Description

CA-5f is a potent late-stage autophagy inhibitor that blocks autophagosome-lysosome fusion. It is a valuable research tool for studying autophagy mechanisms and their roles in cancer and neurodegenerative disease models, both in vitro and in vivo.

Research Area

Cell Biology

Key Properties

• CAS Number: 1370032-19-1
• MW: 388.46
• Purity: 99.83% (May vary between batches)
• Formula: C₂₄H₂₄N₂O₃
• SMILES: COc1ccc(\C=C2/CN(C)C\C(=C/c3c[nH]c4ccccc34)C2=O)cc1OC
• Target: Apoptosis,Autophagy,ATG,p62
• Solubility: DMSO:55 mg/mL (141.58 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.15 mM);H2O:Insoluble

Bioactivity

• In Vivo:
  CA-5f effectively suppressed the growth of A549 lung cancer xenograft as a single agent with an excellent tolerance in vivo. Results from western blot, immunofluorescence, and TdT-mediated dUTP nick end labeling (TUNEL) assays showed that CA-5f inhibited autophagic flux, induced apoptosis, and did not affect the level of CTSB (cathepsin B) and CTSD (cathepsin D) in vivo, which were consistent with the in vitro data.
• In Vitro:
  Treatment of human umbilical vein endothelial cells (HUVECs) with CA-5f for 1 h suppressed the levels of cytoskeletal proteins and membrane traffic proteins. CA-5f exhibited strong cytotoxicity against A549 NSCLC cells, but low cytotoxicity to normal HUVECs, by increasing mitochondrial-derived reactive oxygen species (ROS) production.
• Cell Research:
  A549 cells or HUVECs were seeded into 100-mm culture dishes. When cells reached 80% confluency, they were exposed to DMSO or CA-5f for 6 h or 24 h. Then the cells were fixed, dehydrated, embedded, sectioned, and stained as previously described. Finally, the ultrathin sections of these samples were observed under a JEM-1230 transmission electron microscopy.
• Animal Research:
  Four-week-old BALB/c nude mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Lt and kept in specific pathogen-free conditions at the Experimental Animal Center in the Institute of Medicine in Zhengzhou University. Nude mice underwent subcutaneous injection of A549 cells (1 × 10^6) at the right scapula. 8 days after A549 cell implantation, mice exhibited palpable tumors, then they were randomly divided into 2 groups (each group n = 5). CA-5f was injected via caudal vein every 2 days for up to 30 days. DMSO was used as a control. From the initial injection, the tumor size was measured every 2 days. Tumor volumes were calculated as tumor length × (square of width)/2. At the end of the experiment, all mice were sacrificed and the tumors were resected immediately and photographed. The weight of each tumor was measured.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, Inhibitor, CA5f, CA-5f, CA 5f, Apoptosis, Autophagy

Compound Identifiers

PubChem CID

57341148