CA-5f

SKU: orb1221169

Description

CA-5f is a potent late-stage macroautophagy/autophagy inhibitor via inhibiting autophagosome-lysosome fusion.

Key Properties

• CAS Number: 1370032-19-1
• MW: 388.46
• Purity: >98% (HPLC)
• Formula: C₂₄H₂₄N₂O₃
• SMILES: COc1ccc(\C=C2/CN(C)C\C(=C/c3c[nH]c4ccccc34)C2=O)cc1OC
• Target: Autophagy
• Solubility: DMSO: 70 mg/mL;Water: Insoluble

Bioactivity

• In Vivo:
  Animal model: Nude mice bearing A549 lung cancer cells. Dosage: 40 mg/kg. Administration: Injected via caudal vein, every 2 days for up to 30 days. Result: Significantly suppressed tumor volume and weight in mice, increased the number of apoptotic cells in mice.
• In Vitro:
  Cell Viability Assay Cell line: A549, HUVECs. Concentration: 20 μM. Incubation time: 96 hours. Result: Exhibited more cytotoxicity against A549 cells compared with normal HUVECs. Western blot analysis. Cell line: A549 cells and HUVECs. Concentration: 0-40 μM. Incubation time: 6 hours. Result: Elevated LC3B-II (a marker to monitor autophagy) and SQSTM1 protein levels in a concentration- and time-dependent manner.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Autophagy