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C6orf115 Polyclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Reactivity | Porcine, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Disclaimer | For research use only |
Alternative Names
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Blank control: Mouse spleen.Primary Antibody (green line): Rabbit Anti-C6orf115 antibody.Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488. Dilution: 1 µg /test.Protocol. The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20, 000 events was performed.
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Protocol Information
C6orf115 Polyclonal Antibody (orb1419759)
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