C5AR2/GPR77/C5L2 Antibody

SKU: orb1534145

Description

C5AR2/GPR77/C5L2 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P
• Dilution range: ELISA, IHC, IHC-P (10 µg/ml)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic 20 amino acid peptide from N-Terminus extracellular domain of human C5L2. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Gibbon (95%); Monkey (85%); Marmoset (80%).
• Target: C5AR2 / GPR77 / C5L2
• Purification: Immunoaffinity purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.1% Sodium Azide. PBS, 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

C5AR2, G-protein coupled receptor 77, GPF77, GPR77, C5L2, G protein-coupled receptor 77

Quick Database Links

Gene Symbol

C5AR2 / GPR77 / C5L2