C4A Antibody

SKU: orb1252822

Description

C4A Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, IHC
• Reactivity: Human
• Application Notes:
  IF: 1:50-1:100IHC (FFPE): 1:200-1:400 for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)ELISA: 1-2 µg/mL; order the BSA-free formatThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the C4d antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: C4D204
• Immunogen: Human protein was used as the immunogen for this C4d antibody.
• Target: C4A
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CO4, CPAMD2, Complement C4-A, Acidic complement C4C4, RG, C4S, C4A2, C4A3, C4A4, C4A6, C4AD

Quick Database Links

Gene Symbol

C4A

UniProt

P0C0L4