C4A Antibody

SKU: orb1252822

Description

C4A Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC
• Reactivity: Human
• Application Notes:
  IF: 1:50-1:100IHC (FFPE): 1:200-1:400 for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)ELISA: 1-2 µg/mL; order the BSA-free formatThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the C4d antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: C4D204
• Immunogen: Human protein was used as the immunogen for this C4d antibody.
• Target: C4A
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CO4, CPAMD2, Complement C4-A, Acidic complement C4C4, RG, C4S, C4A2, C4A3, C4A4, C4A6, C4AD

IHC testing of FFPE human kidney transplant tissue (10X) stained with C4d antibody (C4D204).

IHC testing of FFPE human kidney transplant tissue (20X) stained with C4d antibody (C4D204).

IHC testing of FFPE human kidney transplant tissue (40X) stained with Complement 4d/C4d antibody (C4D204).

Quick Database Links

Gene Symbol

C4A

UniProt

P0C0L4