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Catalog Number | orb1257016 |
---|---|
Category | Antibodies |
Description | C21orf33 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 42-268 of human C21orf33 (NP_004640.4). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:10 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 25kDa |
Target | C21orf33 |
UniProt ID | P30042 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | C21orf33, D21S2048E, ES1, GT335, HES1, KNP-I, KNPH Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:10 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using C21orf33 antibody (orb1257016) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
Immunofluorescence analysis of U2OS cells using C21orf33 antibody (orb1257016).
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, WB | |
Canine, Gallus, Mouse, Porcine | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
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