C21orf33 Antibody

SKU: orb1257016

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Description

C21orf33 Antibody

Images & Validation

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Item 1 of 2

Tested Applications	IF, WB
Reactivity	Human, Mouse, Rat
Application Notes	WB: 1:500 - 1:2000IF: 1:10 - 1:100

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 42-268 of human C21orf33 (NP_004640.4).
Target	C21orf33
Molecular Weight	Observed: 25kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid
Concentration	batch dependent
Disclaimer	For research use only

Alternative Names

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C21orf33, D21S2048E, ES1, GT335, HES1, KNP-I, KNPH, KNPI

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Western blot analysis of extracts of various cell lines, using C21orf33 antibody (orb1257016) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Immunofluorescence analysis of U2OS cells using C21orf33 antibody (orb1257016).

Quick Database Links

Gene Symbol

C21orf33

UniProt

P30042

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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