TSPEAR Antibody (Center)

SKU: orb1928009

This product can be custom made

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

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Item 1 of 3

Tested Applications	FC, IHC-P, WB
Dilution Range	WB - 1:1000, IHC-P - 1:50-100, FC - 1:10-50
Reactivity	Human

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	This C21orf29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-320 amino acids from the Central region of human C21orf29. Antigen Region: 293-320 aa.
Target	TSPEAR
Molecular Weight	74924 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Thrombospondin-type laminin G domain and EAR repeat-containing protein, TSP-EAR, TSPEAR, C21orf29

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

C21orf29 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-C21orf29 Antibody (Center) at 1:1000 dilution + 293 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 75 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with C21orf29 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.